Application Guide for the TD-700 Laboratory Fluorometer: A versatile instrument for environmental and biotechnology applications

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TD-700 Laboratory Fluorometer
Application Configuration Guide

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					Optical Kit
Application	Assay Kit/Dye	Instrument P/N	PMT (installed)	Lamp P/N & Description	*Optical Kit P/N	Excitation Filter P/N & Description	Emission Filter P/N & Description	Cuvette/ Test Tube P/N & Material	Adaptor	Solid Secondary Standard
Aflatoxin	Vicam Aflatestä Mycotoxin Testing System (Method P/N 998-2610)	7000-009	Standard 300-650nm	10-049 Near U.V.	7000-967	10-069R 300-400nm	10-110RC 410-610nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor	7000-992
Antibiotic Sensitivity	SYTOX® Green (Method P/N 998-2676)	7000-009	Standard 300-650nm	7000-930 Quartz Halogen	Order Filters from Omega® Optical	Omega® Optical 485DF22	Omega® Optical 530DF30	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor	7000-994
Bacterial Viability	Baclightä , Live/Dead Assay (Method P/N 998-2678)	7000-009	Standard 300-650nm	7000-930 Quartz Halogen	(Order individual filters)	10-105 486nm	10-129 520nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor	7000-993 and 7000-994
Bacterial Viability	Baclightä , Live/Dead Assay (Method P/N 998-2678)	7000-009	Standard 300-650nm	7000-930 Quartz Halogen	(Order individual filters)	10-129 520nm	10-058R 325-700nm 10-054R >610nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor	7000-993 and 7000-994
Chlorophyll a	Extractive (Acidification Method) and In-vivo (Acidification method) (Method P/N 998-2607)	7000-000	Red 185-870nm	10-045 Daylight White	7000-961	10-050R 340-500nm	10-051R >665nm	10-031 13mmx100mm Borosilicate Test Tube or 10-028A 25mmx150mm Borosilicate Test Tube	7000-981 13mmx100mm or 7000-982 25mmx150mm Test Tube Adaptors	7000-994
Chlorophyll a	Extractive (Non-acidification Method)	7000-000	Red 185-870nm	10-089 Blue Mercury Vapor	7000-962	10-113 436nm	10-115 680nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor included with instrument	7000-994
Chlorophyll a	In-Vivo (fresh water, samples with high DOM and/or Chlorophyll b)	7000-000	Red 185-870nm	10-089 Blue Mercury Vapor	7000-963	10-050R 340-500nm	10-115 680nm	10-028A 25mmx150mm Borosilicate Test Tubes	7000-982 25mmx150mm Test Tube Adaptor included with instrument	7000-994
Cell Proliferation	CyQUANTä (Method P/N 998-2672)	7000-009	Standard 300-650nm	10-089 Blue Mercury Vapor or 7000-930 Quartz Halogen	7000-964	10-105 486nm	10-109R-C 510-700nm	7000-959 10mmx10mm Square Methacrylate Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-994
DNA, ds	PicoGreen® (Method P/N 998-2630)	7000-009	Standard 300-650nm	10-089 Blue Mercury Vapor	7000-964	10-105 486nm	10-109R-C 510-700nm	7000-957 10mmx10mm Square Polystyrene Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-994
DNA, ds & ss	Hoechst Dye 33258 (Method P/N 998-7074)	7000-009	Standard 300-650nm	10-049 Near U.V.	7000-967	10-069R 300-400nm	10-110RC 410-610nm	7000-959 10mmx10mm Square Methacrylate Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-992
Enzyme Studies	NADPH	7000-009	Standard 300-650nm	10-049 Near U.V.	7000-967	10-069R 300-400nm	10-110R-C 410-610nm	7000-959 10mmx10mm Square Methacrylate Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-992
Fluorescent Proteins	EBFP (Method P/N 998-2670)	7000-009	Standard 300-650nm	10-049 Near U.V.	(Order individual filters)	10-134 390nm	10-110R-C 410-610nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor	7000-992
Fluorescent Proteins	EGFP (Method P/N 998-2670)	7000-009	Standard 300-650nm	10-089 Blue Mercury Vapor	7000-964	10-105 486nm	10-109R-C 510-700nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor	7000-994
Fluorescent Proteins	EYFP (Method P/N 998-2670)	7000-009	Standard 300-650nm	10-089 Blue Mercury Vapor	7000-964	10-105 486nm	10-109R-C 510-700nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor	7000-994
Fluorescent Proteins	GFPwt (Green Fluorescent Protein) (Method P/N 998-2670)	7000-009	Standard 300-650nm	10-049 Near U.V.	(Order individual filters)	10-134 390nm	10-109R-C 510-700nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor	7000-992
Fluorescent Proteins	GFPuv (Green Fluorescent Protein) (Method P/N 998-2670)	7000-009	Standard 300-650nm	10-049 Near U.V.	(Order individual filters)	10-134 390nm	10-109R-C 510-700nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Test Tube Adaptor	7000-992
Fluorescent Proteins	GFP (Green Fluorescent Protein in Whole Bacteria) (Method P/N 998-7072)	7000-009	Standard 300-650nm	10-049 Near U.V.	(Order individual filters)	10-134 390nm	10-109R-C 510-700nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm Round Test Tube Adaptor	7000-992
Fluorescent Tracer Studies	Fluorescein	7000-009	Standard 300-650nm	10-089 Blue Mercury Vapor	7000-964	10-105 486nm	10-109R-C 510-700nm	10-028A 25mmx150mm Borosilicate Test Tube	7000-982 25mmx150mm Test Tube Adaptor	7000-993
Fluorescent Tracer Studies	Rhodamine WT (Standard PMT)	7000-009	Standard 300-650nm	10-046 Clear Quartz	7000-966	10-103 550nm	10-052R >570nm	10-028A 25mmx150mm Borosilicate Test Tube	7000-982 25mmx150mm Test Tube Adaptor	7000-994
Fluorescent Tracer Studies	Rhodamine WT (Red PMT)	7000-000	Red 185-870nm	10-046 Clear Quartz	7000-966	10-103 550nm	10-058R 325-700nm & 10-052R >570nm	10-028A 25mmx150mm Borosilicate Test Tube	7000-982 25mmx150mm Test Tube Adaptor	7000-994
Fluorescent Tracer Studies	Uvitex Dye	7000-009	Standard 300-650nm	10-049 Near U.V.	(Order individual filters)	10-069R 300-400nm	10-059R and 10-061 (install 10-061 before 10-059R into filter cylinder) 410-500nm	10-031 13mmx100mm Borosilicate Test Tube	7000-981 13mmx100mm	7000-992
General Purpose Fluorophore	4-methylumbelliferyl (b -methylumbelliferone, 7-hydroxy 4-methylcoumarin) (Method P/N 998-2679)	7000-009	Standard 300-650nm	10-049 Near U.V.	7000-967	10-069R 300-400nm	10-110R-C 410-610nm	7000-959 10mmx10mm Square Methacrylate Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-992
General Purpose Fluorophore	FITC	7000-009	Standard 300-650nm	10-089 Blue Mercury Vapor	7000-964	10-105 486nm	10-109R-C 510-700nm	7000-959 10mmx10mm Square Methacrylate Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-993
Histamine	I.N.E.P.A.C.A. Method (Method P/N 998-7076)	7000-009	Standard 300-650nm	10-049 Near U.V.	(Order individual filters)	10-069R 300-400nm	10-059R & 10-061R 410-500nm	7000-959 10mmx10mm Square Methacrylate Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-992
Phosphatase Detection	DiFMUP (Method P/N 998-7075)	7000-009	Standard 300-650nm	10-049 Near U.V.	(Order individual filters)	10-126 365nm	10-110R-C 410-610nm	7000-959 10mmx10mm Square Methacrylate Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-992
Protease	CaspACEä (Method P/N S-0004)	7000-009	Standard 300-650nm	10-049 Near U.V.	(Order individual filters)	10-126 365nm	10-104R-C 455-500nm	500 Microcells included with 7000-951	10mmx10mm Square Cuvette Adaptor included with instrument plus 7000-951 Minicell Adaptor Kit	7000-992
Protease	EnzChekä (Method P/N 998-2601)	7000-009	Standard 300-650nm	10-089 Blue Mercury Vapor	7000-964	10-105 486nm	10-109R-C 510-700nm	7000-957 10mmx10mm Square Polystyrene Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-994
Protein	NanoOrangeä (Method P/N 998-2650)	7000-009	Standard 300-650nm	10-089 Blue Mercury Vapor	7000-964	10-105 486nm	10-109R-C 510-700nm	7000-957 10mmx10mm Square Polystyrene Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-994
Reporter Gene Studies	B-Galactosidase (Method P/N 998-7073)	7000-009	Standard 300-650nm	10-049 Near U.V.	7000-967	10-069R 300-400nm	10-110RC 410-610nm	7000-959 10mmx10mm Square Methacrylate Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-992
RNA	Ethidium Bromide (Method P/N 998-2675)	7000-009	Standard 300-650nm	10-046 Clear Quartz or 7000-930 Quartz Halogen	(Order individual filters)	10-103 550nm	10-052R >570nm	7000-955 10mmx10mm Square Glass Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-994
RNA	RiboGreenä (Method P/N 998-2623)	7000-009	Standard 300-650nm	10-089 Blue Mercury Vapor	7000-964	10-105 486nm	10-109R-C 510-700nm	7000-959 10mmx10mm Square Methacrylate Cuvette	10mmx10mm Square Cuvette Adaptor included with instrument	7000-994